Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

In RNA-seq library preparation, selecting the appropriate number of PCR cycles is a critical balancing act to avoid overcycling.

In the rapidly advancing life science fields, endpoint polymerase chain reaction (PCR), real-time PCR (qPCR), and digital PCR (dPCR) have become indispensa | Ebooks ...

Despite its utility, PCR’s main limitation is that it requires precise cycles of heating and cooling to amplify DNA. The thermal cyclers that perform this operation are clunky, relatively expensive, ...

Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have ...

PCR Biosystems, the UK-based specialists in DNA and RNA analysis, have launched a range of IsoFast™ Bst Polymerase reagents for rapid, robust and sensitive DNA and RNA amplification. Through its ...

PCR enables precise copying of DNA methylation during DNA amplification, overcoming a fundamental limitation of PCR, and unlocks new opportunities for sensitive detection of cancer, chronic disease, ...

Plants have evolved a variety of defense mechanisms against predators and environmental challenges. Among their protective devices are a myriad of compounds, some very complex in structure, that allow ...