Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...

Reduce amplification bias caused by variable miRNA abundance using automated PCR normalization for NEXTFLEX small RNA sequencing workflows.

The virus and prion validation group at Octapharma GmbH (Frankfurt, Germany) reports how implementing an INTEGRA VIAFLO 96 has enabled them to reduce the time taken to undertake PCR assays by greater ...

High-resolution melting has proven to be a highly sensitive method for mutation discovery. PCR is performed in the presence of the double-strand DNA binding dye LCGreen® Plus. Samples are then ...