Molecular analysis using PCR techniques demands both precision and versatility, integrating methods that offer unique yet complementary strengths. However, researchers can face challenges balancing ...
When DNA targets high in GC-content are amplified, PCR product formation can often be compromised by inadequate strand separation and the propensity for complex secondary structure formation. The use ...
Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...
Today, PCR is ubiquitous in molecular biology laboratories. As one of the first experiments that young scientists master, it is easy to take for granted. But developing this research lab staple ...
Microzone in the United Kingdom has recently introduced microLYSIS, a reagent designed to lyse a wide variety of different cell types prior to PCR. MicroLYSIS is a proprietary mixture of detergents ...
Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have ...
When carrying out conventional PCR workflows users have to specify how many amplification cycles they need according to input quantity and assay type. This rigid structure is particularly difficult ...
Isothermal amplification methods streamline nucleic acid amplification without the need for thermal cycling. Join us to explore two innovative techniques, Recombinase Polymerase Amplification (RPA) ...
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