Here we present Primer3Plus, a new web interface to the popular Primer3 primer design program as an enhanced alternative for the CGI- scripts that come with Primer3. Primer3 consists of a command line ...

We are trying to get the same primer output from primer3_core using as what we got on primer3plus. However, I tried to apply the same setting as we used on primer3plus and got different results. I ...

I am using Primer3Plus version 3.3.0 and I am frequently running into problems when trying to design primer pairs with internal hybridization oligos. To me it seems impossible to set a Hyb Oligo ...