Real-time PCR (qPCR) is the gold-standard for sensitive, specific detection and quantification of nucleic acid targets through fluorescence-based techniques. TaqMan assays and SYBR Green dye are two ...
The development of the polymerase chain reaction (PCR) in 1983 by Kary Mullis and coworkers revolutionized molecular biology, allowing scientists to amplify DNA sequences for various applications. 1 ...