リアルタイムポリメラーゼ連鎖反応法（PCRまたはqPCR）は、ポリメラーゼ連鎖反応（PCR）に基づく分子生物学的検査手法です。リアルタイムPCRでは、従来のPCRのようにPCRプロセスの最後ではなく、その途中で特定のDNA分子の増幅をモニターします ...

全世界に蔓延する牛伝染性リンパ腫ウイルス（BLV）からウシを守る第一歩 ――革新的迅速遺伝子診断法 BLV-CoCoMo Dual qPCRの液体試薬と乾燥試薬の開発―― 【発表のポイント】 牛伝染性リンパ腫ウイルス(BLV)のlong terminal repeat(LTR)領域と宿主遺伝子(BoLA-DRA遺伝 ...

舌スワブを用いた高感度qPCR法を開発。多施設評価で感度は87.4％、特異度は98.0％ 舌スワブを用いたqPCR法は、痰採取が困難な場合の肺結核診断に有望な代替手段 喀痰採取は一苦労なので、再現性確認されれば助かる Wang, Yilin, Junwei Cui, Yuanyuan Li, Miao Wang, Wenge ...

In the rapidly advancing life science fields, endpoint polymerase chain reaction (PCR), real-time PCR (qPCR), and digital PCR (dPCR) have become indispensa | Ebooks ...

The systems are available on both PIPETMAX® 268 , Gilson's established compact liquid handler, and the new PIPETMAX® 278 , a larger, more flexible platform built for higher throughputwith expanded ...

For qPCR studies as well as for final data processing and analysis, it is essential to known the primer amplification efficiency (Livak and Schmittgen, 2001; Pfaffl, 2001), since it determines which ...

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The composition of the cheese microbiome has an important impact on the sensorial quality and safety of cheese. Therefore, much effort has been made to investigate the microbial community composition ...

Introduction HIV coinfection presents a challenge for diagnosis of visceral leishmaniasis (VL). Invasive splenic or bone marrow aspiration with microscopic visualisation of Leishmania parasites ...

RT-dPCR is analytically more sensitive than RT-qPCR for the trace detection of SARS-CoV-2 in wastewater. This will be vital for wastewater surveillance in low-prevalence COVID-19 areas. In this study, ...